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1.
J Prosthodont Res ; 2024 Feb 22.
Artigo em Inglês | MEDLINE | ID: mdl-38383001

RESUMO

PURPOSE: This study aimed to determine the usefulness of machine learning techniques, specifically supervised and unsupervised learning, for assessing the cementation condition between a fixed partial denture (FPD) and its abutment using a resonance frequency analysis (RFA) system. METHODS: An in vitro mandibular model was used with a single crown and three-unit bridge made of a high-gold alloy. Two cementation conditions for the single crown and its abutment were set: cemented and uncemented. Four cementation conditions were set for the bridge and abutments: both crowns were firmly cemented, only the premolar crown was cemented, only the molar crown was cemented, and both crowns were uncemented. For RFA under cementation conditions, 16 impulsive forces were directly applied to the buccal side of the tested tooth at a frequency of 4 Hz using a Periotest device. Frequency responses were measured using a 3D accelerometer mounted on the occlusal surface of the tested tooth. Both supervised and unsupervised learning methods were used to analyze the datasets. RESULTS: Using supervised learning, the fully cemented condition had the highest feature importance scores at approximately 3000 Hz; the partially cemented condition had the highest scores between 1000 and 2000 Hz; and the highest scores for the uncemented condition were observed between 0 and 500 Hz. Using unsupervised learning, the uncemented and partially cemented conditions exhibited the highest anomaly scores. CONCLUSIONS: Machine learning combined with RFA exhibits good potential to assess the cementation condition of an FPD and hence facilitate the early diagnosis of FPD retention loss.

2.
Jpn Dent Sci Rev ; 60: 73-80, 2024 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-38298267

RESUMO

Unreasonable medical fees can cause problems such as increased medical costs, greater medical disparities, decreased medical standards, and physician shortages. To prevent such problems, it is important to set appropriate medical fees, ensure their proper use, and improve the efficiency of medical care. The treatment of patients with maxillofacial defects is generally more expensive compared with general prosthodontic treatment because it involves more materials and requires more frequently follow-ups for longer period. However, the actual time required for maxillofacial prosthetic treatment is unclear. Therefore, in this study, we aimed to clarify the amount of time spent treating maxillofacial prosthetic patients. We analyzed clinical data from patients undergoing routine maxillofacial prosthetic treatment, irrespective of difficulty level, at 8 university hospitals and 2 dental clinics. We also collected data from maxillofacial prosthodontists on the treatment time required for various Japanese health insurance items, including the fabrication of maxillofacial prostheses. The results revealed that some aspects of maxillofacial prosthetic treatment may take longer to perform and are more costly to perform than previously thought, suggesting the need for some adjustments to the health insurance reimbursement system. Maintaining an appropriate balance between expenditures and fees will greatly benefit patients and physicians, ensuring positive health outcomes and a healthy society.

3.
J Oral Sci ; 66(1): 30-36, 2024 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-38030286

RESUMO

PURPOSE: Using multivariate analysis, this study investigated the effectiveness of maxillofacial prosthetic treatment in relation to oral health-related quality of life (OHR-QoL), masticatory ability (food intake status score and gum-jelly test score) and related factors in patients who had undergone surgery for head and neck tumors. METHODS: The study cohort comprised 112 patients who underwent surgical resection and prosthetic treatment among 224 patients with head and neck tumors seen at the Maxillofacial Prosthetics Clinic of Tohoku University Hospital in a 2-year period. Correlations between OHR-QoL, food intake status score, and gum-jelly test score (criterion variables), and age, sex, maxillary defect, tongue/soft tissue defect, reconstructive surgery, and chemoradiotherapy (explanatory variables) were investigated, and the data were examined statistically. RESULTS: Maxillary defect, tongue and soft palate tissue defect, and chemoradiotherapy were identified as factors that hindered the effectiveness of maxillofacial prosthetic treatment for improvement of the OHR-QoL, food intake status score, and gum-jelly test score. On the other hand, reconstructive surgery was a factor that facilitated the improvement of OHR-QoL and masticatory ability with maxillofacial prosthetic treatment. CONCLUSION: The factors identified to be related to the success or failure of maxillofacial prosthetic treatment suggest the importance of combining prosthetic intervention with surgical reconstruction.


Assuntos
Neoplasias de Cabeça e Pescoço , Procedimentos de Cirurgia Plástica , Humanos , Qualidade de Vida , Neoplasias de Cabeça e Pescoço/cirurgia , Maxila/cirurgia
4.
Regen Ther ; 25: 49-60, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38089138

RESUMO

Introduction: During tissue repair or regeneration, several bioactive molecules are released and interact with each other and act as complex additives or inhibitors for tissue reconstruction. In this study, the bone-healing effects of the combination treatment with tumor necrosis factor-α (TNF-α) inhibition, vascular endothelial growth factor A (VEGF-A) and bone morphogenetic protein-7 (BMP-7) release by gene silencing, and gene transfection with calcium phosphate nanoparticles (CaP) in the rat femoral head was histologically, morphologically, and biochemically evaluated. Methods: A triple-functionalized paste of CaP carrying plasmid DNA encoding for BMP-7 and for VEGF), and siRNA against TNF-α was developed and denoted as CaP3mix. To compare the effects of 3mixCaP, CaP with plasmid DNA encoding BMP-7, VEGF, or siRNA encoding TNF-α was prepared and denoted as CaP/PEI/pBMP-7/SiO2, CaP/PEI/pVEGF/SiO2, or CaP/PEI/siRNA-TNF-α/SiO2, respectively. The bone healing in bone defects in the rat femoral head was investigated after 10 and 21 days of implantation. Results: The levels of bone formation-related markers OCN, Runx2, and SP7 increased at the protein and gene levels in 3mixCaP after 10 days, and 3mixCaP significantly accelerated bone healing compared with the other treatments after 21 days of implantation. Conclusion: The triple-functionalized CaP paste loading plasmid DNA encoding BMP-7 and VEGF and siRNA encoding TNF-α is a promising bioactive material for bone tissue repair.

6.
Dysphagia ; 2023 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-37752277

RESUMO

The risk of dysphagia and/or aspiration is determined using screening tests, such as the repeated saliva swallowing test and modified water swallowing test, which evaluate cued swallowing. However, humans masticate and swallow foods with various consistencies, forms, and amounts, without conscious awareness. Therefore, this study aimed to examine the difference in the behavior of masticatory and swallowing muscles during spontaneous versus cued swallowing through a series of mastication and swallowing processes by evaluating surface electromyogram (sEMG) signals. The effect of the consistency and amount of food on the behavior of these muscles was also investigated. The sEMG recordings of the masseter muscles and anterior belly of the digastric muscle for 12 subjects, and genioglossus muscle for 5 subjects were obtained. The genioglossus activity was recorded using custom-made ball electrodes. The test foods were cookies and tofu, in amounts of 2 g and 4 g. The normalized muscle activity (integrated EMG), duration of the muscle activity, initial activation timepoint of each muscle, and total duration of swallowing were compared among four conditions. The activity of each muscle was significantly higher during the swallowing of cookies than tofu, for 4 g vs 2 g, and for cued versus spontaneous swallowing. The duration of each muscle activity, initial activation timepoint, and total duration of swallowing were significantly longer for cookies versus tofu, for 4 g vs 2 g, and for spontaneous versus cued swallowing. These results suggest that the behavior of the masticatory and swallowing muscles is affected by cued swallowing and by the consistency and amount of food.

7.
Radiat Prot Dosimetry ; 199(14): 1620-1625, 2023 Sep 18.
Artigo em Inglês | MEDLINE | ID: mdl-37721074

RESUMO

The signal intensities of CO2- radicals in teeth can be utilised as an individual indicator of the cumulative external dose for animals. To accurately determine the external dose, it is desirable to analyse the CO2- radical intensity and improve its detection limit. We recently reported a dose-response in the range of 0-200 mGy and estimated the absorbed dose for seven wild Japanese macaques captured in/around the related areas to the Fukushima Daiichi Nuclear Power Plant accident. Herein, for further improvement of this method, we examined the electron spin resonance spectra of the teeth of these seven and an additional four macaques captured in Fukushima by applying two spectrum-decomposition algorithms.


Assuntos
Acidente Nuclear de Fukushima , Macaca fuscata , Animais , Dióxido de Carbono , Espectroscopia de Ressonância de Spin Eletrônica , Algoritmos
8.
Medicine (Baltimore) ; 102(37): e35066, 2023 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-37713859

RESUMO

Surgical site infections (SSI) are associated with increased morbidity and mortality rates. This study aimed to investigate the ability of perioperative oral management (POM) to reduce the risk of SSI in abdominal surgery Real-world data collected from 16 university hospitals in Japan were reviewed. The medical records of consecutive 2782 patients (1750 men and 1032 women) who underwent abdominal surgery under general anesthesia at 16 university hospitals were retrospectively reviewed. Detailed information about SSI was assessed and compared between patients with and without POM in univariate and multivariate analyses. SSI were observed in 275 patients (incidence rate:9.9%), and POM was administered to 778 patients (28.0%). Univariate analyses revealed that diabetes mellitus, Eastern Cooperative Oncology Group performance status, American Society of Anesthesiologists classification, surgical site, preoperative Prognostic Nutritional Index score, POM, extent of surgery, operation time, and intraoperative blood loss were significantly associated with postoperative SSI (Chi-square or Mann-Whitney U test, P < .01). Multivariate analysis revealed that POM had significant preventive effects against postoperative SSI (estimate: -0.245, standard error: 0.080, P < .01). Surgical site, American Society of Anesthesiologists classification, and operation time were also significant and independent clinical predictors of SSI. The analysis of real-world data from 16 university hospitals revealed that, regardless of the content and degree of the problem, the addition of POM has significant beneficial effects in reducing the risk of SSI in patients who undergo abdominal surgery. Medical records from each hospital and data from the Health Care Payment Fund were collected and analyzed retrospectively.


Assuntos
Infecção da Ferida Cirúrgica , Masculino , Humanos , Feminino , Infecção da Ferida Cirúrgica/epidemiologia , Infecção da Ferida Cirúrgica/prevenção & controle , Japão/epidemiologia , Estudos Retrospectivos , Universidades , Hospitais Universitários
9.
J Prosthodont ; 2023 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-37767904

RESUMO

PURPOSE: The optimal configuration of a customized implant abutment plays a crucial role in promoting bone remodeling and maintaining the peri-implant gingival contour. However, the biomechanical effects of abutment configuration on bone remodeling and peri-implant tissue remain unclear. This study aimed to evaluate the influence of abutment taper configurations on bone remodeling and peri-implant tissue. MATERIALS AND METHODS: Five models with different abutment taper configurations (10°, 20°, 30°, 40°, and 50°) were analyzed using finite element analysis (FEA) to evaluate the biomechanical responses in peri-implant bone and the hydrostatic pressure in peri-implant tissue. RESULTS: The results demonstrated that the rate of increase in bone density was similar in all models. On the other hand, the hydrostatic pressure in peri-implant gingiva revealed significantly different results. Model 10° showed the highest maximum and volume-averaged hydrostatic pressures (69.31 and 4.5 mmHg), whereas Model 30° demonstrated the lowest values (57.83 and 3.88 mmHg) with the lowest excessive pressure area. The area of excessive hydrostatic pressure decreased in all models as the degree of abutment taper increased from 10° to 30°. In contrast, Models 40° and 50° exhibited greater hydrostatic pressure concentration at the cervical region. CONCLUSION: In conclusion, the abutment taper configuration had a slight effect on bone remodeling but exerted a significant effect on the peri-implant gingiva above the implant platform via hydrostatic pressure. Significant decreases in greatest and average hydrostatic pressures were observed in the peri-implant tissues of Model 30°. However, the results indicate that implant abutment tapering wider than 40° could result in a larger area of excessive hydrostatic pressure in peri-implant tissue, which could induce gingival recession.

10.
BMC Oral Health ; 23(1): 695, 2023 09 27.
Artigo em Inglês | MEDLINE | ID: mdl-37759198

RESUMO

BACKGROUND: This study aimed to evaluate the efficacy and safety of dentin hypersensitivity (DH) treatment using a newly developed device based on a powder jet deposition (PJD) technique that creates a hydroxyapatite (HAP) layer on the dentin surface, thereby alleviating the hypersensitivity. The effect of the PJD treatment was compared with that of conventional treatment using Teethmate Desensitizer (TMD; calcium-phosphate containing material with TTCP (Ca4(PO4)2O) and DCPA (CaHPO4)), which has been used clinically in Japan with well-confirmed effectiveness. MATERIALS AND METHODS: A randomized controlled trial was conducted including 35 patients who had symptoms of DH in two or more quadrants. Two test teeth were selected per patient (70 teeth in total) and randomly assigned to PJD or TMD treatment. The efficacy was evaluated using the improvement rate for air and scratch pain according to the scores obtained via visual analog scale 12 weeks after treatment. The safety assessment was performed focusing on gingival index (GI) and spontaneous pain. The t-test was used to analyze the non-inferiority of PJD treatment compared to TMD treatment. RESULTS: The improvement rate of air pain was 69.0% for PJD and 69.7% for TMD. The improvement rate of scratch pain was 80.8% for PJD and 81.7% for TMD. Non-inferiority with a margin of 10% was not observed for both air and scratch pain. No change was observed in GI from baseline and the improvement rate of spontaneous pain for PJD was higher than that for TMD. CONCLUSION: Non-inferiority of PJD to TMD treatment was not observed in this study; however, it was not statistically demonstrated, and the results were thus interpreted as inconclusive. PJD did improve the DH symptoms, as did TMD. PJD's therapeutic effect was most likely attributable to the deposition of a HAP layer on the tooth surface, which would alleviate hypersensitivity for at least 12 weeks without causing severe adverse events. TRIAL REGISTRATION: UMIN-CTR. ID: UMIN000025022. date: 02/12/2016.


Assuntos
Sensibilidade da Dentina , Pós , Humanos , Sensibilidade da Dentina/terapia , Durapatita/uso terapêutico , Japão , Dor , Pós/uso terapêutico
11.
Int J Implant Dent ; 9(1): 31, 2023 09 19.
Artigo em Inglês | MEDLINE | ID: mdl-37725286

RESUMO

BACKGROUND: Appropriate load distribution among the supporting elements is essential for the long-term success of implant-assisted removable partial dentures; however, there is little information available on load distribution. PURPOSE: This study aimed to evaluate the effect of implant location on load distribution in implant-assisted removable partial dentures by reviewing in vitro models and finite-element analysis studies. MATERIALS AND METHODS: English-language studies which examined the load distribution of implant-assisted removable partial dentures and were published between January 2001 and October 2022 were extracted from PubMed, ScienceDirect, and Scopus online databases, and manual searching. Two reviewers selected the articles based on the predetermined inclusion and exclusion criteria, followed by data extraction and analysis. RESULTS: Forty-seven studies were selected after evaluating the titles and abstracts of 264 articles; two were identified manually. After screening the text, 12 studies were included: six in vitro model experiments and six finite-element analysis studies. All included studies used a mandibular free-end missing model (Kennedy Class I or II). The influence of implant location on load distribution to the abutment tooth, implant, and mucosa under the denture base was summarized in three cases: implant at the premolar, first molar, and second molar region. Due to differences in the measurement method of load distribution and loading condition to the denture, the results differed among the studies. CONCLUSIONS: The implant location in implant-assisted removable partial dentures can affect load distribution to the supporting elements, such as the abutment tooth, implant, and mucosa under the denture base.


Assuntos
Implantes Dentários , Prótese Parcial Removível , Dente Molar , Bases de Dados Factuais , Análise de Elementos Finitos
12.
Front Oral Health ; 4: 1170966, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37521175

RESUMO

Background: Orofacial pain conditions are complex disorders that involve biological, social, and psychological factors. Temporomandibular Disorders (TMDs) are one of the most common orofacial pain conditions, and our previous literature review indicated that exercise therapy has shown promise in reducing TMD-related pain. However, more evidence is needed to firmly establish its effectiveness. Objectives: This systematic review aims to investigate the effectiveness of exercise therapy on pain relief and jaw mobility in patients with pain-related TMDs. Methods: To include randomized controlled trials (RCTs) written in English, a literature search was performed using PubMed, Scopus, Web of Science, Cochrane Library, Ovid, EBM reviews, and Academic Search Complete initially from 4th November 2020 until March 2022. A PICOS for this review was as follows; P: Patients with TMD myalgia or arthralgia, I: Excursion exercise, Stretch exercises, Resistance exercise, or Coordination exercise, C: No treatment or education only. O: Pain intensity and Range of Motion (ROM), S: RCTs. After title screening, a full-text assessment was done to extract data. According to Risk of Bias (RoB) 2.0, risk of bias was assessed in each included paper by 2 reviewers independently. Results: A total of 3,388 titles were identified from the electronic database search. After the screening and full-text evaluation, only 5 studies (145 participants) were eligible to be included. Among the exercise modalities, coordination exercise showed a significant effect on pain relief and improvement of joint mobility. Discussion: Due to the heterogeneity and small sample size of the included studies, a meta-analysis was not feasible. However, this systematic review suggested that exercise therapy, especially coordinate exercise, can be effective in managing painful TMD conditions. Further research is needed to establish optimal parameters for this patient population, as well as standardization and consistency in terminology and treatment structure.

13.
J Prosthet Dent ; 130(3): 380.e1-380.e9, 2023 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-37482534

RESUMO

STATEMENT OF PROBLEM: Titanium base (TiBase) abutments to restore an implant-supported single crown are available in different gingival heights, but information on the biomechanical effects of the gingival heights is lacking. PURPOSE: The purpose of this nonlinear finite element analysis study was to evaluate the effects of TiBase gingival heights on the biomechanical behavior of custom zirconia (CustomZir) abutments and TiBase, including von Mises stress and maximum and minimum principal stress. MATERIAL AND METHODS: TiBases with different gingival heights (0.5 mm, 1 mm, 1.5 mm, and 2 mm) with internal hexagon Morse taper connections were simulated in 3-dimensional models. The simulations (ANSYS Workbench 2020; ANSYS Inc) included the OsseoSpeed EV implant (Ø5.4 mm) (AstraTech; Dentsply Sirona), restoration, and surrounding bone in the mandibular first molar region. An occlusal force of 200 N was applied with a 2-mm horizontal offset toward the buccal side and a 30-degree inclination from the vertical axis. RESULTS: High-stress concentration was observed in the uppermost internal connection area on the buccal side and the antirotational part of the titanium abutment on the lingual side in all models. CustomZir abutments with a shorter gingival height exhibited larger concentrated areas of volume average stress von Mises stress and higher magnitude of maximum and minimum principal stress compared with a taller gingival height. CONCLUSIONS: A TiBase abutment with a taller gingival height reduced the fracture risk of a CustomZir abutment without increasing any mechanical risk.


Assuntos
Implantes Dentários para Um Único Dente , Implantes Dentários , Projeto do Implante Dentário-Pivô , Titânio , Análise de Elementos Finitos , Estresse Mecânico , Dente Suporte , Análise do Estresse Dentário , Fenômenos Biomecânicos
14.
J Dent Sci ; 18(2): 840-847, 2023 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-37021257

RESUMO

Background/purpose: The SimEx is dental training system that applies new technology in a computerized dental simulator. The purpose of this study was to understand the usability satisfaction of the SimEx dental education and evaluation system by dental students and dentists at Tohoku University. Materials and methods: In this study, the Tohoku University IRB execution number was 2020-3-33. The number of subjects accepted was 59 at Tohoku University and divided into 4 groups based on years of clinical experience (Group A: 0 years; Group B: 1-2 years; Group C: 2-5 years; Group D: at least 5 years), and a total of 58 usability questionnaires were collected. Subjects completed the SimEx Usability Satisfaction Questionnaire after operating the SimEx (EPED Inc., Kaohsiung, Taiwan) course, which contained 16 questions. Results: Among the 58 questionnaires collected by Tohoku University, there were 19 undergraduate students (4th∼6th grade), 12 post-graduate students, 14 residents, and 13 dentists. Significant differences between Group A and Group B, and between Group A and Group D were found (P < 0.05). The same results were obtained for the "experience satisfaction index". In the items where significant differences were found, longer clinical experience tended to result in lower scores. Conclusion: From these results, we can conclude that the SimEx education and evaluation system facilitates students' self-learning, and this system is very useful for continued study and clinical skill training for dentists, especially for students and junior dentists with high usability satisfaction.

15.
Leg Med (Tokyo) ; 62: 102234, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-36933509

RESUMO

This study aimed to examine the applicability of the pulp/tooth ratio (PTR) method for age estimation in Mongolian populations using panoramic radiographs and derive new regression formulae. Moreover, we aimed to assess the accuracy of these formulae in other subjects from the Mongolian population and compare them with the other formulae derived from different Asian populations. The total sample size of the study was 381. The formulae were derived from the examination of panoramic radiographs of 271 individuals aged 15-62 years. Following Cameriere's method, PTR was calculated for the upper and lower canine teeth. Linear regression analyses were performed between the actual age and that obtained from upper-lower canine PTR and established formulae for age estimation. To verify the formulae, two types of test samples were collected: 73 panoramic radiographs and 37 periapical radiographs. The estimated age was calculated using our new formulae and three other formulae derived from Asian populations. The correlation coefficient between the actual age and that obtained by PTR was significantly negative for both canines. According to our new regression formulae, the differences between the estimated age and actual age showed a bell-shaped curve distribution in both test groups. While using the other formulae derived from the Asian population, the distribution patterns obtained were significantly different in the Mongolian population. This study was the first to examine the relationship between actual age and PTR in Mongolian population, and these results advance the field of forensic science in Mongolia.


Assuntos
Determinação da Idade pelos Dentes , Humanos , Determinação da Idade pelos Dentes/métodos , Radiografia Dentária Digital , Análise de Regressão , Povo Asiático , Odontologia Legal/métodos
16.
J Environ Radioact ; 258: 107103, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36599216

RESUMO

To elucidate 137Cs contamination levels and mechanisms of fish inhabiting river and pond environments near the Fukushima Dai-ichi Nuclear Power Plant, 137Cs activity concentrations in fish (15 species, n = 164) and water collected from Maeda River (3.3-8.9 km from the plant) and Shimofukazawa Pond (2.9 km) in 2017 were analyzed. Also, an 8-week rearing experiment using Japanese dace Pseudaspius hakonensis fed on non-contaminated pellets and the pond water (mean 137Cs concentration of 2.0 Bq/L) was conducted to evaluate 137Cs accumulation from water to fish. The 137Cs concentrations in Japanese dace, the only species collected throughout five sampling sites from estuarine to upstream areas in Maeda River, were found to be correlated with ambient air dose rates and fish size, exhibiting large variations (16.5-2.6×103 Bq/kg-wet). By contrast, dissolved 137Cs in river waters increased from the upper to lower course (0.025-0.28 Bq/L), which caused large variations of the water-to-body concentration ratio (CR) in Japanese dace (60.0-35700 L/kg-wet). These CRs (geometric mean of 3670 L/kg-wet) were much higher than the steady-state CR of reared fish (9.7 L/kg-wet), indicating that river fish uptake 137Cs mainly from prey items from aquatic and riparian zones, rather than from water. Statistically significant negative correlations between K+ concentrations in water and river fish CRs were detected, resulting in the decreasing trend of CRs from upstream to estuarine areas. These results suggest that the large heterogeneity of air dose rates, K+ concentration, and estuarine processes in brackish water habitats, in association with the feeding habit and size effect in fish, can engender wide variation of 137Cs concentrations and CRs of river fish along a river course. In contrast, 137Cs concentrations in pond fish (4.3-14.6 kBq/kg-wet) were higher than in river fish. The CRs of pond fish were constantly high but the range was smaller (1010-3440 L/kg-wet) with larger values in fish of higher trophic levels. These findings suggest that biomagnification within a pond was inferred as the main cause of 137Cs contamination of pond fish.


Assuntos
Cipriniformes , Acidente Nuclear de Fukushima , Monitoramento de Radiação , Radioatividade , Poluentes Radioativos da Água , Animais , Lagoas , Centrais Nucleares , Radioisótopos de Césio/análise , Água , Poluentes Radioativos da Água/análise , Japão
17.
Regen Ther ; 22: 99-108, 2023 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-36712960

RESUMO

Introduction: Periodontal ligament is regenerated in association with hard tissue regeneration. Tenomodulin (Tnmd) expression has been confirmed in periodontal ligament and it reportedly inhibits angiogenesis or is involved in collagen fibril maturation. The introduction of Tnmd by gene transfection in bone tissue regeneration therapy might inhibit topical hard tissue formation and induce the formation of dense fibrous tissue. Therefore, the effect of Tnmd introduction by gene transfection technique in vitro and in vivo was investigated in this study. Methods: Osteogenesis- and chondrogenesis-related gene expression levels in osteoblastic cells (MC3T3E1) and rat bone marrow derived cells were detected using qPCR three days after gene transfection with plasmid DNA (Tnmd) using non-viral gene transfection vectors: a calcium phosphate-based gene transfection vector (CaP(Tnmd)) or a cationic polymer-based reagent (JetPEI (Tnmd)). Next, an atelocollagen scaffold with or without CaP (Tnmd) or JetPEI (Tnmd) was implanted into a rat calvaria bone defect, and the remaining bone defect volume and the tissue reaction at 28 days after surgery were evaluated. Results: Runx 2 and SP7 mRNA was reduced by JetPEI (Tnmd) in both cells, but not in CaP(Tnmd). The volume of expressed Tnmd was at 9 ng/mL in both gene transfection vector. The remaining bone defect volume of JetPEI (Tnmd) was significantly bigger than that of the other groups and CaP (EGFP), and that of CaP (Tnmd) was significantly bigger than that of CaP (EGFP). Conclusions: Tnmd introduction treatment inhibits bone formation in artificial bone defect, however, the effect of that was dependent on non-viral gene transfection vector.

18.
J Prosthet Dent ; 129(5): 754-762, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-34364689

RESUMO

STATEMENT OF PROBLEM: As players of single-reed wind instruments such as the clarinet and saxophone are likely to experience pain and trauma of the lower lip, a method to prevent these symptoms without disturbing the music quality and performance is required. PURPOSE: The purpose of this clinical study was to produce a custom music splint, an oral appliance for the players of single-reed wind instruments, by using sports mouthguard sheet materials and to determine its suppression effect on the pressure and pain of the lower lip and teeth and on fatigue and pain of the jaw. MATERIAL AND METHODS: The soft type and soft and hard 2-layer type of sheet materials for sports mouthguards were used to fabricate the music splints. Each type of material has 1-mm and 2-mm thicknesses; therefore, 4 kinds of music splint were produced. The participants, students who played wind instruments in university orchestras, were instructed to use each music splint when playing the instruments for 1 week (for at least 3 hours for each music splint) and to answer a questionnaire about the effect of each appliance. Eighteen participants answered the questionnaire. Based on the usual usage of oral appliances when playing instruments, the participants were classified into 2 groups; oral appliance typically used (group OA) (n=7) and oral appliance not used (group noOA) (n=11). To determine the effects of the 4 types of music splint, a multilevel linear regression analysis was applied to evaluate the nested structure of the data (α=.05). RESULTS: The use of the music splint significantly relieved pressure and pain on the lower lips. The soft type was more effective in group OA, and the thin type was more effective in group noOA. The thin- and soft-type music splints were associated with significantly better playing sensation and sound quality than other types of splint in both groups. CONCLUSIONS: The thin- and soft-type music splints can be effective and useful for suppressing oral symptoms when playing a single-reed wind instrument without disturbing the quality of the performance.


Assuntos
Música , Dente , Humanos , Contenções , Dureza , Dor
19.
J Prosthodont Res ; 67(2): 189-195, 2023 Apr 12.
Artigo em Inglês | MEDLINE | ID: mdl-35644569

RESUMO

PURPOSE: To investigate the effect of prosthodontic treatment on the ingestible food profile in adult Japanese outpatients, and to identify the related risk factors that can deteriorate the profile. METHODS: The participants were 277 outpatients who visited university-based specialty clinics in Japan for prosthodontic treatment. The demographic data, number of present teeth assessed via intraoral examination, and oral health-related quality of life assessed by the total Oral Health Impact Profile (OHIP-J54) scores of all participants were recorded before treatment. Ingestible food profile score (IFS) was recorded using a validated food intake questionnaire. Eligible participants who answered the questionnaire before and after treatment were categorized into five groups based on the prosthodontic treatments they received (i.e., crowns, bridges, removable partial dentures, removable complete dentures, and removable complete and partial dentures). RESULTS: Multivariate analysis of covariance revealed a statistically significant main effect of prosthodontic intervention (time course: before and after treatment) on mean IFS (P=0.035, F=4.526), even after adjusting for covariates (age, number of present teeth, and treatment modality). Multiple linear regression analysis revealed that the low number of present teeth (r=0.427, P<0.001) and a high OHIP-J54 total score (r=-0.519, P<0.001) of the patients at the baseline were significantly associated with their baseline IFSs, even after adjusting for confounding variables. CONCLUSIONS: The findings of this multicenter follow-up study indicate the importance of prosthodontic rehabilitation in improving patients' ingestible food profiles.


Assuntos
Prótese Parcial Removível , Qualidade de Vida , Adulto , Humanos , População do Leste Asiático , Seguimentos , Saúde Bucal , Pacientes Ambulatoriais , Prostodontia , Alimentos , Dieta
20.
Int J Prosthodont ; 36(2): 181-188, 2023 May.
Artigo em Inglês | MEDLINE | ID: mdl-33662065

RESUMO

PURPOSE: To evaluate the effect of different abutment screw retightening procedures on the screw stability between the abutment and implant. MATERIALS AND METHODS: External hexagon connection (EHC) and internal trichannel connection (ITC) implants were employed in this study. Each abutment screw was used for the following specific applications (n = 5 for each group): group 0 = abutment was tightened and retightened with a 10-minute interval; group 1 = abutment was tightened and retightened with a 10-minute interval, then loaded for 500,000 cycles; group 2 = abutment was loaded for 83,000 cycles during tightening and retightening, then loaded for 500,000 cycles; group 3 = abutment was loaded for 167,000 cycles during tightening and retightening, then loaded for 500,000 cycles; and group 4 = abutment was loaded for 250,000 cycles during tightening and retightening, then loaded for 500,000 cycles. Both tightening and retightening torques were set at 35 Ncm, and dynamic oblique loading between 0 N and 200 N was applied on the abutment in all groups. The settling values of the abutments after retightening and the abutment removal torque values (RTVs) after testing were measured. RESULTS: No significant differences in settling values were found between EHC and ITC. There were significant differences in the RTVs of EHC implants, but ITC implants showed no difference in RTV with different prosthetic retightening applications. CONCLUSION: The retightening application affected the joint stability of EHC implants but did not affect the settlement and joint stability of ITC implants. Int J Prosthodont 2023;36:181-188. doi: 10.11607/ijp.6863.


Assuntos
Projeto do Implante Dentário-Pivô , Implantes Dentários , Dente Suporte , Análise do Estresse Dentário , Parafusos Ósseos , Torque
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